First birth of a healthy infant following intra-cytoplasmic sperm injection using a new permeable cryoprotectant-free sperm vitrification protocol
نویسندگان
چکیده
منابع مشابه
Sperm retrieval for intra-cytoplasmic sperm injection in non-obstructive azoospermia.
Surgical testicular sperm retrieval for intra-cytoplasmic sperm injection (ICSI) purposes is the only possibility of biological fathering in case of non-obstructive azoospermia (NOA). Successful retrieval only correlates with histology, not with FSH values or testicular volume. Concurrent AZFa and AZFb microdeletion predict unsuccessful recovery. Testicular sperm extraction (TESE) (mean of succ...
متن کاملP-56: Artificial Oocyte Activation Alone Intra Cytoplasmic Sperm Injection in Men with Severe Semen Parameters
Background Failed fertilization occurs 1-3% in the infertile couples. Failure of oocyte activation (OA) is mainly attributed to failed fertilization. ICSI alone artificial oocyte activation (AOA) is only treatment approach for these individuals. Three procedures are introduced for AOA: chemical, mechanical and electrical. Most of the clinical Centers have commonly used ionomycin for activation ...
متن کاملRelationship between Potential Sperm Factors Involved in Oocyte Activation and Sperm DNA Fragmentation with Intra-Cytoplasmic Sperm Injection Clinical Outcomes
OBJECTIVE The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development. MATERIALS AND METHODS In this experimental study,...
متن کاملHuman Sperm Aster Formation and Chromatin Configuration in Rabbit Oocytes Following Intracytoplasmic Sperm Injection Using a Piezo-Micromanipulator
In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human ...
متن کاملSperm-induced oocyte activation in the rhesus monkey: nuclear and cytoplasmic changes following intracytoplasmic sperm injection.
Intracytoplasmic sperm injection (ICSI) has increased the potential of the assisted reproductive technologies to propagate mammalian species and has provided an opportunity for research into cell cycle control and the mechanisms involved in sperm-induced oocyte activation. We have investigated the efficacy of ICSI in the rhesus monkey, the mechanism of fertilization following sperm injection an...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 2019
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2019.01.014